rabbit cyclind1 polyclonal antibody  (Boster Bio)

Journal: Scientific Reports

Article Title: DLGAP4 acts as an effective prognostic predictor for hepatocellular carcinoma and is closely related to tumour progression

doi: 10.1038/s41598-022-23837-y

Figure Lengend Snippet: Interfering with DLGAP4 inhibits the PPARβ/δ signalling pathway and the expression of proliferation- and metastasis-related proteins. Western blotting was performed to measure the protein expression of DLGAP4, PPARβ/δ, CyclinD1, E-cadherin and N-cadherin in shNC or shDLGAP4 HepG2 and HCCLM3 cells. The data were obtained from the average of three independent experiments. *P < 0.05.

Article Snippet: The following antibodies were used: rabbit PPARβ/δ monoclonal antibody (1:2000, Abcam, Cambridge, MA, USA), mouse β-actin polyclonal antibody (1:1000, Boster Biological Technology, Ltd), rabbit E-cadherin polyclonal antibody (1:1000, Boster Biological Technology, Ltd), rabbit N-cadherin polyclonal antibody (1:1000, Boster Biological Technology, Ltd), rabbit DLGAP4 polyclonal antibody (1:1000, Abcam, Cambridge, MA, USA), and rabbit CyclinD1 polyclonal antibody (1:1000, Boster Biological Technology, Ltd.).

Techniques: Expressing, Western Blot

Journal: Frontiers in Medicine

Article Title: Keratin 17 Suppresses Cell Proliferation and Epithelial-Mesenchymal Transition in Pancreatic Cancer

doi: 10.3389/fmed.2020.572494

Figure Lengend Snippet: K17 induced cell cycle arrest and cell apoptosis in pancreatic cancer cells in vitro . (A) Cell cycle distribution was analyzed by flow cytometry in stable K17 silenced SW1990 and CFPAC-1 cells and stable K17 upregulated HPDE6-C7 and PANC-1 cells. (B) Flow cytometry was used to determine the percentage of apoptotic cells in stable K17-silenced SW1990 and CFPAC-1 cells and in stable K17 upregulated HPDE6-C7 and PANC-1 cells. (C) Western blot analysis was performed to determine the expression of CyclinD1 and cleaved Caspase3 protein in stable K17-silenced SW1990 and CFPAC-1 cells and in stable K17 upregulated HPDE6-C7 and PANC-1 cells. * P < 0.05, and ** P < 0.01.

Article Snippet: The membranes were incubated at 4°C overnight with the following antibodies: rabbit monoclonal K17 (ab109725, Abcam, Cambridge, MA, UK), rabbit polyclonal cleaved Caspase3 (ab2302, Abcam, Cambridge, MA, UK), rabbit polyclonal CyclinD1 (26939-1-AP, ProteinTech Group, Inc., Wuhan, China) and rabbit polyclonal GAPDH (10494-1-AP, ProteinTech Group, Inc., Wuhan, China).

Techniques: In Vitro, Flow Cytometry, Western Blot, Expressing

Journal: The Journal of Biological Chemistry

Article Title: Forkhead Box Protein 1 (FoxO1) Inhibits Accelerated β Cell Aging in Pancreas-specific SMAD7 Mutant Mice *

doi: 10.1074/jbc.M116.770032

Figure Lengend Snippet: β Cell dysfunction and aging in SMAD7Ptf1a mice. A–G, body weight (A), fasting blood glucose (B), IPGTT (C and D), serum insulin (E), β cell mass (F), and serum glucagon (G) at different ages in SMAD7Ptf1a mice compared with littermate control SMADfx/fx mice. H, 1 week of BrdU labeling followed by quantification of the percentage of β cells that incorporated BrdU. I and J, enrichment of gene transcripts of CyclinD1 (I) and CyclinD2 (J) in mouse islets of various ages. The values were normalized against CycloA, which is consistent among all conditions. K, islet perifusion study using islets from 20-week SMAD7Ptf1a and SMAD7fx/fx mice. Impaired glucose-stimulated insulin secretion was detected in SMAD7Ptf1a mice. *, p < 0.05 and n = 5 in all cases.

Article Snippet: Primary antibodies were as follows: guinea pig polyclonal insulin-specific (Dako, Carpinteria, CA); goat polyclonal NeuroD1-specific (Santa Cruz Biotechnology, Dallas, TX); rabbit polyclonal SMAD7-, CyclinD1-, and CyclinD2-specific (Santa Cruz Biotechnology); FoxO1- and GAPDH-specific (Cell Signaling Technology, San Jose, CA), Collagen I- and Pdx1-specific (Abcam, Cambridge, MA, USA), MafA-specific (Bethyl Laboratories, Inc.); Nkx6.1-specific (a kind gift from Dr. Maike Sander, University of California, San Diego, CA); and rat CD31-specific (BD Biosciences) and BrdU-specific (Abcam).

Techniques: Control, Labeling